After injury concerns, Venus Williams loses to Jelena Ostapenko in the Birmingham Classic.

The second-seeded Jelena Ostapenko defeated the 43-year-old American, who is currently ranked No. 697, on Thursday in the second round of the grass-court competition that is serving as a warm-up for Wimbledon. The score was 6-3, 5-7, 6-3.

At the Birmingham Classic, Venus Williams was unable to pull off a larger shock.

Williams’ victory over Camile Giorgi, who was ranked 48th, on Monday marked her first over a player in the top 50 in over four years. Her hamstring injury, which she got in Auckland during the opening week of the year, forced her to take six months off.

Williams received an off-court medical timeout at 2-2 in the second set while Ostapenko was wearing strapping around her right knee. She seemed uncomfortable as soon as she walked out on the grass, but she persisted in playing, fighting for every point, and even saving a match point at 5-3 before coming back to win four straight games and force a decision.

Williams’ mobility had decreased by the end of the third set, which lasted 44 minutes, and Ostapenko won with a forehand down the line.

Williams, a seven-time Grand Slam winner and five-time Wimbledon singles champion, was given a wild card this week by the All England Club for this year’s championships, and she has earned it.

Top-seeded Earlier, Barbora Krejcikova kept up her certain progress by defeating fellow Czech player Tereza Martincova in straight sets to go to the quarterfinals. Krejcikova has now gone the entire event without dropping a set after winning 6-4, 6-4. The No. 12-ranked Krejcikova defeated Cristina Bucsa in the opening round 6-3, 6-3.

Magdalena Frech of Poland defeated eighth-seeded Sorana Cirstea of Romania 6-3, 6-7 (1), 6-4, and Rebecca Marino of Canada defeated Emina Bektas of America, who was rated 150th, 6-4, 4-6, 7-6 (1).

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